Simple, accurate and reproducible RP-HPLC method for simultaneous estimation of salbutamol (SAL) and ambroxol (AMB) was developed by using Thermo C18 column (4.6 mm i.d × 250 mm) and mobile phase acetonitrile (0.025M) and potassium dihydrogen orthophosphate buffer (pH adjusted to 3.0 with orthophosphoric acid) in the ratio of 30:70% v/v. Linearity was observed in the range of 5-25µg/ml for salbutamol and 10-50µg/ml for ambroxol. The drugs were subjected to oxidation, hydrolysis and heat to apply stress condition for degradation studies. Results of analysis were validated statistically and by recovery studies. Hence it can be concluded that developed RP-HPLC method are accurate, precise and selective, can be employed for the estimation of salbutamol and ambroxol in bulk as well as formulation.
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